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Hairpin
Primer sequences (multiplex PCR primers supported,
example
) *
Number of primer sequences: 1 (min) - 50 (max). 2 (max) primers for degenerate bases.
Cutoff
Minimum size (minus gap) of double helix to trigger a hairpin.
Temperature
°C
Temperature for thermodynamics calculation
Concentration of monovalent cations (usually KCl) *
mM
Concentration of divalent cations (usually MgCl
2
) *
mM
Concentration of dNTPs *
mM
Annealing oligo concentration *
nM
Download
MFEprimer-3.0
Specificity
Hairpin
Dimer
Virtual Electrophoretogram
Database Require
Database List