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Dimer
Primer sequences (multiplex PCR primers supported,
example
) *
Number of primer sequences: 1 (min) - 50 (max). 2 (max) primers for degenerate bases.
Score cutoff
Minimum score value required for a dimer.
Maximum mismatch
How many mismatches allowed in a dimer?
3'end key bases
In default, the last 3 bases at the 3'end are treated differently and there should be zero mismatches among these bases.
Maximum mismatch allowd in 3'end key bases
How many mismatches allowed in 3'end key bases in a dimer?
Gap/mismatch run
Maximum continuous mismatches allowed in a dimer.
Temperature
°C
Temperature for thermodynamics calculation.
Concentration of monovalent cations (usually KCl) *
mM
Concentration of divalent cations (usually MgCl
2
) *
mM
Concentration of dNTPs *
mM
Annealing oligo concentration *
nM
Download
MFEprimer-3.0
Specificity
Hairpin
Dimer
Virtual Electrophoretogram
Database Require
Database List